Editing Vitis rotundifolia (section)

== Propagation ==

Softwood cuttings under mist are a standard propagation route in muscadine production, while hardwood rooting is more variable. A two-year University of Arkansas greenhouse study tested hardwood cuttings approximately 15–20 cm long and 5–10 mm thick, with three nodes, in perlite and 0.1% IBA at 18–24 °C with assessment at 90 days. Its results and discussion identify collection date, storage, bottom heat, cane position, genotype, and source vineyard as relevant factors; the tested conditions do not establish one hardwood recipe for every cultivar or grower.<sup id="cite_ref-4">[[#cite_note-4|[4]]]</sup>

Seed propagation is useful for breeding and for producing variable seedlings rather than preserving a named vine. In University of Georgia experiments with open-pollinated Fry seed, 90 days of stratification at 4 °C gave the highest germination among the tested stratification periods, and alternating 32/22 °C germination outperformed the tested alternatives. Hydrogen peroxide and low-rate gibberellic acid helped in some treatments, whereas high gibberellic acid was detrimental; the results were treatment-specific and did not fully reproduce an earlier report of more than 90% germination.<sup id="cite_ref-5">[[#cite_note-5|[5]]]</sup>